Innovative solutions

Biofilm detection and removal 

NEW TBF 300®

Biofilms detector

Specifically designed to detect biofilms quickly and easily on surface based on their selective staining. 

NEW packaging, more practical and effective.


Results in a few minutes


Does not require specialised technicians


The reagent reacts in a selective way with the biofilm matrix, preventing the risk of false positives.

Use and application of TBF® 300

TBF® 300 is a quick test to detect biofilms on surfaces. This product allows identifying biofilm contamination areas in a quick, simple, and selective way.

In addition, it is an effective tool for the routine hygienic control of surfaces and the cleaning and disinfection procedures.

It comes in a 125 ml bottle with spray to facilitate application on surfaces.

1. Choose a test sampling area and make sure it is representative of the surface to be analysed. It is recommended to apply the product over an area of at least 10 cm on a horizontal surface.

2. Apply the product by pressing the spray so the surface is covered in an even way.

3. Leave the product to work for at least 5 minutes.

4. Rise the surface with water and check for the presence of any residual colouring. Residual colouring (fuchsia) after rinsing indicates the potential presence of biofilms.

The product includes a strong colouring capacity.

It must be applied in small doses and in a controlled way, using gloves to prevent contact with the skin.

Do not apply the product over porous surfaces that may retain the die permanently (wood, cloth, cardboard, etc.). If in doubt, it is recommended to carry out a preliminary test on a sample of the surface.

Also, there may be a large amount of die in the rinsing water and it could stain any absorbent materials it comes into contact with.

TBF® 300 contains biological staining agents selected because of their ability to detect biofilms formed by different bacterial species.

The biofilm staining can be seen with the naked eye, with no need for specific instruments or specialised technicians.

The detector effectively stains biofilms formed by bacteria from the Pseudomonas fluorescens, Bacillus cereus, Staphylococcus aureus, Salmonella enterica y Listeria monocytogenes, among others.

The staining agents die in an irreversible way that exopolymeric matrix components of biofilms, so the colouring is not specific of the bacterial species present in the biofilm.

The colourants in the TBF® 300 have also been tested for the typical waste present in food industries to prevent unwanted interferences. TBF® 300 does not stain residues such as milk, fat, or lean meat.

The product contains surfactant, thickening and stabilising agents to facilitate application and retention over the surface.

TBF® 300 is also soluble in water, so it can be removed by rinsing with water, except in those places where a biofilm has been formed.

* Requerido Email * Nombre * Apellidos * Empresa * Pa’s * United States of America Afghanistan Albania Algeria Andorra Angola Antigua And Barbuda Argentina Armenia Aruba Australia Austria Azerbaijan Bahamas Bahrain Belarus Belgium Belize Bolivia Bosnia and Herzegovina Brazil British Indian Ocean Territory Brunei Darussalam Bulgaria Canada Cape Verde Central African Republic Chile China Colombia Costa Rica Croatia Cuba Cyprus Czech Republic Democratic Republic of the Congo Denmark Dominican Republic Ecuador Egypt El Salvador Equatorial Guinea Estonia Ethiopia Finland France Georgia Germany Gibraltar Greece Guatemala Haiti Honduras Hong Kong Hungary Iceland India Indonesia Iran Iraq Ireland Israel Italy Jamaica Japan Jordan Lebanon Libya Liechtenstein Lithuania Luxembourg Macedonia Madagascar Malaysia Maldives Malta Mexico Monaco Mongolia Montenegro Morocco Mozambique Netherlands Netherlands Antilles New Zealand Nicaragua Nigeria Norway Pakistan Palestine Panama Papua New Guinea Paraguay Peru Philippines Poland Portugal Puerto Rico Qatar Romania Russia San Marino Saudi Arabia Serbia Seychelles Singapore Slovakia Slovenia Somalia South Africa South Korea Spain Sudan Sweden Switzerland Syria Taiwan Tanzania Thailand Tunisia Turkey Ukraine United Arab Emirates United Kingdom Uruguay USA Minor Outlying Islands Venezuela Vietnam He le’do y acepto la Pol’tica de Privacidad.

Biofilms removal

The MIDA® range of products degrade the biofilm matrix, causing its detachment and allowing the removal of the microorganisms contained.


Effective removal of biofilms.


Combination of chemical and enzymatic technology.


High biocide capacity and broad spectrum.

Chemical removal

MIDA® SAN 327 BF and MIDA® SAN 328 EC  are part of a product range with biocide properties, intended to remove biofilms from surfaces.


Foaming disinfectant with biofilm removing properties for applications in the food industry.

Garrafa 21 kg.

IBC 1000 kg.


Non-foaming disinfectant with biofilm removing properties for applications in the food industry.

Garrafa 21 kg.

IBC 1000 kg.

Enzymatic removal

MIDA® ADD 409 AG is an additive with a combination of 6 different enzymes that, when used together with detergents such as MIDA® FOAM 152 SA, facilitates the biofilms removal.


MIDA® ADD 409 AG is a liquid additive containing a mixture of different enzymes, including proteases, lipases, amylases and others, which contribute to degrading the protective matrix of the biofilm.

Canister 20 kg

IBC 1000 kg.


Foaming detergent with a medium alkalinity, used in combination with the additive, to remove the biofilm matrix.

Canister 25 kg.

IBC 1000 kg.

About us


Polígono Industrial Raconc, Parcelas nº 2 y 3

46729 Ador (Valencia, España)

Telephone +34 962 871 345

Fax: +34 962 875 867

©2020 BETELGEUX-CHRISTEYNS. All rights reserved