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TBF® 300

The BIOFILM DETECTION TEST TBF 300 is a rapid test for detection of microbial biofilms on surfaces. This product has been developed as a simple and fast technique for the selective identification of bioifilms, and is the result of several years of research in collaboration with Universidad Complutense de Madrid (Spain).

The BIOFILM DETECTION TEST TBF 300 is employed for assessing the presence of biofilms on surfaces after the cleaning and disinfection operations, by its application on several sampling points.

1. Select the surface to be analyzed and apply TBF 300 foam over an area of approximately 10 cm2.

2. Allow a contact time of at least 5 minutes.

3. Rinse the test area with water to remove the foam.

4. Check for the presence of pink coloration remaining on the surface after rinsing.

Remaining pink coloration on the test surface indicates the presence of biofilms. Should this be the case, it is recommended to clean and disinfect the surface using biofilm-removing products such as BETELENE BF31 (Betelgeux).

— Do not apply on porous surfaces which may permanently retain the dye (wood, fabric, cardboard, etc.). In case of doubt, apply a small amount of foam on a sample of the surface.

— The use of gloves during application is recommended to prevent staining the skin, which may be difficult to remove.

— Rinsing water may contain amounts of dye large enough to stain non-suitable materials. Always check that this water is conducted over non-absorbing materials.

Each can of BIOFILM DETECTION TEST TBF 300 allows to test around 300 sampling points (150 mL can, 10 cm2 test area).

The components of TBF 300 foam selectively dye the biofilm exopolymeric matrix, without interferences from most residues commonly found in food or cosmetic industries.

This test provides qualitative information on the presence of biofilms on surfaces under study, and it does not replace official methods to assess the sanitary conditions of the facilities. It is necessary to use more specific analysis techniques to obtain data regarding the nature of the biofilm, its constituents or microbiological contamination levels.